CloneID: 7c6
Antigen Long Description: Murine antibodies were produced by immunising BALB/c mice with residues 686-696 (SSINEEITPRRQS) of human BCR fused to thyroglobulin. Hybridomas were generated by fusion of immunised cells with the myeloma cell line P3X63Ag8 via established methods.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the SSINEEITPRRQS epitope of BCR (residues 686-696), and as such is able to recognise full-length BCR and the p210 form of the BCR-ABL fusion protein. p210 BCR-ABL is a cytogenetic marker of chronic myelogenous leukaemia, resulting from gross chromosomal translocation resulting in the 'Philadelphia chromosome'. The fusion protein is a constitutively active tyrosine kinase, causing deregulated downstream signalling and increasing likelihood of oncogenic transformation.
Application Notes (Clone): It has been shown by Western blot that 7c6 binds wildtype, full-length BCR and the p210 BCR-ABL fusion protein.
Deutsch
English
