CloneID: 12G10
Heavy Chain modification: Fc Silent™
Antigen Long Description: This antibody was raised by immunising BALB/C mice with purified β1 integrins from HT-1080 fibrosarcoma cells
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P05556
Specificity Statement: This antibody recognises a ligand-induced binding site on the βA domain of β1 integrin, and thus reacts preferentially with α5β1 integrins in the ligand-activated state. Binding of this antibody alters the position of the α1 helix of β1 integrin, and results in further integrin activation.
Application Notes (Clone): This antibody reacts with β1 integrins in Western blotting (Mould et al, 1995). ELISA analysis has revealed the ability of this antibody to enhance the interaction between β1 integrin and its ligand, fibronectin (Mould et al, 1995). Indeed, activation of integrins by this antibody reduced the induction of osteosarcoma growth and inhibition of NF-κB caused by hERG1 knockdown in osteosarcoma cells (Zeng et al, 2016). This antibody has been used to specifically label ligand-bound cell surface β1 integrins both in immunohistochemical analysis (Izumi et al, 2015; Chao & Kunz, 2009), including in situ proximity ligation (PLA) analysis (Nevo et al, 2010), and in FACS analysis (Brandt et al, 2009).
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