Description: Calprotectin is a heteroduplex of Protein S100-A8/A9 (also called MRP-8/14, or Calgranulin A/B). This antibody recognizes the 14 kDa B component (Protein S100-A9) of the complex. Protein S100-A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. Altered expression of this protein is associated with the disease cystic fibrosis. This antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Conjugate: CF488A
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% BSA, 0.05% azide
Clone: MAC387
Immunogen: Affinity purified monocyte membrane preparation
Antibody Reactivity: Calprotectin/S100-A9
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Histochem Cytochem (1987) 35(11): 1217-1226 (IP; WB; IHC, FFPE)
- J Histochem Cytochem (1990) 38(11): 1669-1675. (IHC, FFPE)
- J Leukocyte Biol (1994) 55: 259. (WB; IHC)
- World J Gastroenterol (2017)23(8): 1477-1488. (Flow, intracellular)
Entrez Gene ID: 6279 (S100A8/Calgranulin A/MRP-8) & 6280 (S100A9/Calgranulin B/MRP-14)
Expected AB Applications: Flow (intracellular) (published for clone)/IP (published for clone)/WB (published for clone)
Z-Antibody Applications: Flow, intracellular (published)/IHC, FFPE (verified)/IP (published)/WB (published)
Verified AB Applications: IHC (FFPE) (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Immunohistology formalin-fixed 0.5-1 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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