CloneID: T11D7e
Antigen Long Description: This antibody was raised by immunising CBA mice with xenogeneic rat thymocytes, which express the Thy-1.1 antigen.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P01830
Specificity Statement: This antibody binds murine and rat CD90. While CD90 is a monomorphic determinant in rats, CD90 is polymorphic in mice. As a result, this antibody reacts with Thy1.1-expressing mice, including AKR and FVB mice, but not Thy1.2-expressing mice, such as CBA and BALB/c.
Application Notes (Clone): This antibody has been used in indirect immunofluorescence analysis of Thy-1 antigen expression in both methanol-fixed and living cell cultures (Dulbecco et al, 1979; Hasthorpe, 1980). This antibody has also been used, in conjunction with rabbit complement, to deplete Thy1-expressing fibroblasts from serum samples through complement-mediated lysis (Mahanthappa et al, 1996).
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