CloneID: DH8.3
Antigen Long Description: This antibody was raised by immunising BALB/c mice with a synthetic peptide, known as 79E (LEEKKGNYVVTDHC), corresponding to the junctional sequence of the Δ2-7 EGFR deletion mutation.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P00533
Specificity Statement: This antibody is specific for ΔEGFR (EGFR type III), a truncated form of the epidermal growth factor receptor that is commonly associated with cancer cells, especially glioblastoma cells. ΔEGFR lacks exons 2–7 of the external domain. This antibody does not cross-react with full-length EGFR.
Application Notes (Clone): This antibody has been used in fluorescence-activated cell sorting, Western blot and immunohistochemical analysis, using acetone-fixed frozen sections, of a tumour panel (Jungbluth et al, 2003). It does not stain normal tissues, which express wild-type EGFR (Jungbluth et al, 2003), and preferentially stains tumours expressing mutant receptors (Hills et al, 1995). This antibody has also been used in immunoprecipitation experiments (Hills et al, 1995) and ELISA analysis (Johns et al, 2002). This antibody has been shown to induce rapid internalisation of truncated EGFR by target cells (Johns et al, 2002), which could have therapeutic applications, given the tumour-restricted expression of ΔEGFR and its role in tumorigenesis.
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