CloneID: 16.43
Antigen Long Description: HA tag is derived from the HA-molecule corresponding to amino acids 98-106 (YPYDVPDYA).
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric goat antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: 16.43 reacts specifically with C-terminal HA-tag.
Application Notes (Clone): HA-tag has been used extensively as a general epitope tag in expression vectors, which can be recognised by 16.43 for detection, isolation and purification of recombinant proteins that have been engineered to express the HA-tag. 16.43 has been used to stain for receptor expression in NK cells (Aust 2009).