CloneID: CTD 4H8
Antigen Long Description: Murine antibodies were produced by immunising mice with a peptide consisting of 10 repeats of the RNA polymerase II C-terminal domain sequence YSPTSPS. In each repeat, Ser5 was phosphorylated. Immunised cells were fused with the Sp2/0-Ag14 myeloma cell line to produce a hybridoma.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P24928
Specificity Statement: CTD 4H8 is known to be reactive with human and S. cerevisiae RNA polymerase II CTDs, however may also be cross-reactive with this protein in other species, including mouse, rat and chicken. The Ab is also reactive with both the phosphorylated and unphosphorylated form of the CTD YSPTSPS repeat. RNAPII is responsible for the transcription of most protein-coding genes in the human genome. The CTD of the enzyme, featuring these YSPTSPS repeats, is crucial for the regulation of the enzyme in a spatial (i.e. positionally along genes) and temporal (i.e. rate of RNA polymerisation) manner.
Application Notes (Clone): This mAb has been used in a variety of methods, including Western blotting of the unphosphorylated (Engelhardt et al, 2005) or Ser5-CTD phosphorylated form (Stock et al, 2007), and in precipitation experiments either simply pulling down RNAPII (Kukalev et al, 2005; Peng et al, 2014) or coupled to massively parallel DNA sequencing (ChIP-seq) of the total RNAPII-associated DNA isolated from human CD4+ cells (Zhang et al, 2012).
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