The iLite® TNF-alpha Xcel Assay Ready Cells provide a means of directly quantifying both the potency and neutralizing antibody response to TNF-alpha antagonists of diverse structure in the same assay, making it an outstanding tool for biosimilar development.
Measuring the functional activity of TNF-alpha, the assay can be used for both potency and immunogenicity assessments of TNF-alpha inhibitors in the same assay and the iLite TNF-alpha Xcel Assay Ready Cells also provide a means of directly comparing a biosimilar and innovator product in the same assay.
The iLite TNF-alpha cells are engineered cells for use in a normalized reporter gene bioassay for screening of anti-TNF alpha activity in samples. The assay is a simple and easy test format which detects anti-TNF alpha activity and, indirectly, the presence of anti-drug antibodies.
The iLite TNF-alpha Xcel Assay Ready Cells are engineered cells, optimized to be responsive to TNF-alpha, resulting in a proportional expression of Firefly Luciferase. Binding of TNF-alpha to its receptor results in activation of the NFkB regulated Firefly luciferase reporter gene construct. iLite TNF-alpha Xcel Assay Ready Cells also contain the Renilla Luciferase reporter gene, under the control of a constitutive promoter.
FOR RESEARCH USE ONLY
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