Clone: OX-34
Specificity: Suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue. May not be suitable for intracellular staining on some cell types. Clone MRC OX-34 recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control. Test results have shown that the antibody is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
Antibody Modification: FITC
Presentation: PBS, 0.09% Sodium Azide, 1% BSA
Immunogen: Activated rat T-helper cells.
Gene: Isotype Control
Description: Antibody LS-C292348 is a mouse IgG2a isotype control. Validated for Flow.
Usage: It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagent. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Recommended Storage: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.
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