Specificity: Suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue. May not be suitable for intracellular staining on some cell types. Clone MRC OX-34 recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control. Test results have shown that the antibody is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
Antibody Modification: Unconjugated
Presentation: PBS, 0.09% Sodium Azide, 1% BSA
Immunogen: Activated rat T-helper cells.
Gene: Isotype Control
Description: Antibody LS-C292343 is a mouse IgG2a isotype control. Validated for Flow.
Usage: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. It is recommended that the user titrates the antibody for use in their system to a concentration equivalent to that of their test reagents.
Recommended Storage: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Concentration: 0.1 mg/ml
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