Formulation: 100 µl of protein A-affinity purified monoclonal antibody.
Immunogen: A peptide corresponding to detyrosinated α-tubulin
Clone Designation: RM444
Shelf life (days): 365
Notes: Detyrosinated α-tubulin is a form of the cytoskeletal protein α-tubulin that has been post-translationally modified by the tubulin carboxypeptidases vasohibin 1 (VASH-1) and VASH-2, which remove the C-terminal tyrosine from α-tubulin.{61815} Detyrosination of α-tubulin occurs in the cytosol and exposes two consecutive glutamate residues that can be further processed to form Δ2- or Δ3-tubulin. Detyrosinated α-tubulin monomers can be re-tyrosinated by tubulin tyrosine ligase and be incorporated into microtubules again. α-Tubulin detyrosination is associated with, but not sufficient for, increased stability of the microtubule and is involved in a variety of biological processes, including neuronal development, β-oxidation, mitosis, and cardiomyocyte contraction.{61816,61817,61815,61818} Increased levels of detyrosination are associated with tumor development in vitro, have been found in tumor tissue from patients with breast cancer, and are positively correlated with breast cancer aggressiveness.{61819,61820} Cayman’s Detyrosinated α-Tubulin Rabbit Monoclonal Antibody can be used for immunocytochemistry (ICC), immunofluorescence (IF), and Western blot (WB) applications.
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