Caspase 4 Activity Detection Substrate for Flow Cytometry

Tested Sample Types: Cell samples

Type: Apoptosis/Caspase

Assay Time: 30 min

Detection Type: Fluorometric method;Flow cytometry

Test Principle: The Caspase 4 Activity Detection Substrate for Flow Cytometry are a novel fluorogenic dye that couple the caspase 4 recognition sequence (LEVD) to a high affinity DNA dye, which have cell membrane permeability and can penetrate the plasma membrane into the cytoplasm. The substrate itself has no fluorescence and has a charge exclusion effect with DNA. During cell apoptosis, caspase-4 cleaves the substrate and releases high affinity DNA dyes, which bind to DNA to produce strong fluorescence, thereby detecting caspase-4 activity and visualizing the morphological changes of the cell nucleus during apoptosis.

• SKU: ELSE-CK-A484-100
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: E-CK-A484-100
• Package Unit: 100 Tests
• Quantity Unit: PAK