Clone: LT27
Format: Pacific Blue™
Regulatory Status: RUO (GMP)
Concentration: 0.020 mg/ml
Storage Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage / Stability: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Description: SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Product Specific References: *Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/26785729,*Kralickova P, Milota T, Litzman J, Malkusova I, Jilek D, Petanova J, Vidlakova J, Zimulova A, Fronkova E, Svaton M, Kanderova V, Bloomfeld M, Parackova Z, Klocperk A, Haviger J, Kalina T, Sediva A: CVID-associated tumors: Czech nationwide study focused on epidemiology, immunology, and genetic background in a cohort of patients with CVID. Frontiers in Immunology 2019, 9:3135, URL: ,
General References: *Yun LW, Decarlo AA, Hunter N: Blockade of protease-activated receptors on T cells correlates with altered proteolysis of CD27 by gingipains of Porphyromonas gingivalis. Clin Exp Immunol. 2007 Nov;150(2):217-29., URL: https://www.ncbi.nlm.nih.gov/pubmed/17937677?ordin,*Kara IO, Sahin B, Gunesacar R: Expression of soluble CD27 and interleukins-8 and -10 in B-cell chronic lymphocytic leukemia: correlation with disease stage and prognosis. Adv Ther. 2007 Jan-Feb;24(1):29-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/17526459?ordin,*Molica S, Vitelli G, Levato D, Crispino G, Dell'Olio M, Dattilo A, Matera R, Gandolfo GM, Musto P. CD27 in B-cell chronic lymphocytic leukemia. Cellular expression, serum release and correlation with other soluble molecules belonging to nerve growth factor receptors (NGFr) superfamily. Haematologica. 1998 May;83(5):398-402., URL: https://www.ncbi.nlm.nih.gov/pubmed/9658722?ordina,*van Oers MH, Pals ST, Evers LM, van der Schoot CE, Koopman G, Bonfrer JM, Hintzen RQ, von dem Borne AE, van Lier RA: Expression and release of CD27 in human B-cell malignancies. Blood. 1993 Dec 1;82(11):3430-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/8241510?ordina,
Contents: 1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
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