NADPH/NADP Ratio Assay Kit

Shelf life (days): 365

Info: Cayman’s NADPH/NADP Ratio Assay Kit provides a colorimetric method for measuring NADP+, NADPH, and their ratio in cell culture extracts. In this assay, NADP+ is enzymatically converted to NADPH by glucose-6-phosphate dehydrogenase (G6PDH), which leads to the reduction of the tetrazolium salt substrate WST-8 to a highly colored formazan that absorbs at 450 nm. The amount of formazan produced is proportional to the amount of NADP+ and NADPH in the matrix. To measure NADPH specifically, samples are heated to degrade NADP+. The NADP+ concentration is then calculated by subtracting the signal obtained from the heated sample from that of the corresponding non-heated sample. This assay has a range of 0-1.5 µM and a lower limit of detection (LLOD) of 0.02 µM.

Assay Range: 0-1.5 µM

Formula Weight: 0

• SKU: CAY702450-96
• Manufacturer: Cayman Chemical
• Manufacturer SKU: 702450-96
• Package Unit: 96 Well
• Quantity Unit: PAK