Product Description: Interleukin-17A (IL-17A), also known as CTLA-8, is a member of the IL-17 family of proteins. IL-17A is a proinflammatory cytokine that is secreted by activated CD4+ and CD8+ T lymphocytes. IL-17A acts through its receptor, IL-17R, to promote increased cytokine and chemokine secretion. In turn, the cytokines and chemokines mediate the immunoregulatory function of IL-17A by promoting the proliferation, maturation, and chemoattraction of neutrophils to inflammatory sites. Elevated levels of IL-17A are associated with rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer, and multiple sclerosis. Human, mouse, and rat IL-17A show activity on mouse cells.
Source: E. coli
Purity: >95% determined by reducing and non-reducing SDS-PAGE
Formulation: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Endotoxin Level: <1.00 EU/ug as measured by kinetic LAL
Molecular Weight: 15.1 / 30.2 kDa
Stability and Storage: 12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.
Species: Mouse
Activity Assay: Mouse IL-17A is fully biologically active when compared to standard. The activity is determined by the ability to produce mouse IL-6 by NIH-3T3 cells and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2 x 105 units/mg.
AA Sequence: MAAIIPQSSA CPNTEAKDFL QNVKVNLKVF NSLGAKVSSR RPSDYLNRST SPWTLHRNED PDRYPSVIWE AQCRHQRCVN AEGKLDHHMN SVLIQQEILV LKREPESCPF TFRVEKMLVG VGCTCVASIV RQAA
Length: 134 / 268 aa
Structure: Monomer
Accession Number: Q62386
Reconstitution: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
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