Immobilized soybean trypsin inhibitor

Immobilized soybean trypsin inhibitor, 10 ml
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Molecular Weight: 21500
Storage Buffer: 0.1M Tris-HCl; 0.15M NaCl; 0.02% Na Azide; pH 7.4
Source: Soybean
Purity: >95% by SDS-PAGE analysis
Gene: KTI3
Swiss ID: P01070
Description: Soybean trypsin inhibitor (SBTI) is a reversible competitive inhibitor of trypsin and other trypsin-like proteases such as chymotrypsin, plasmin and plasma kallikrein. It has a MW of 22 kDa and a pI of 4.5 (1,2). We have immobilized SBTI on an agarose resin via coupling to primary amines to create an affinity resin with specificity for a number of serine proteases. SBTI binds free trypsin tighter with increasing pH from 4.5-8.0 that allows for a simple gradient to low pH (~2.5-3.0) for elution (1). References: 1. Birk, Y. (1976) Methods Enzymol. 55:700-706. 2. Kassell, B. (1970) Methods Enzymol. 19:853-862.
Mehr Informationen
Artikelnummer MOISBTI-I-10
Hersteller Molecular Innovations
Hersteller Artikelnummer SBTI-I-10
Verpackungseinheit 10 ml
Mengeneinheit FL
Reaktvität Soy Bean
Human Gene ID 547831
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