Active human uPA functional assay ELISA kit

Active human uPA functional assay ELISA kit, 1 kit
Artikelnummer
MOIHUPAKT-1
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Source: Multiple
Target: Urokinase
Gene: PLAU
Swiss ID: P00749
Description: Urokinase plasminogen activator (uPA), along with its receptor uPAR, is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion and migration. Clinical studies have indicated that high uPA levels may elevate the risk for tumor invasion and metastasis. Increased expression and activity can exert potent arthritogenic properties in rheumatoid arthritis patients. Increased uPA activity may be an implication for the pathophysiology of endometriosis. The sensitive quantitative measurement of functionally active human uPA in plasma and other biological fluids is easily performed with this 96 well strip format ELISA kit. The mean value of uPA in healthy donors was found to be 1.1 ng/ml. The assay measures active uPA in the 0.1-50 ng/ml range. Samples giving human uPA levels above 50 ng/ml should be diluted in blocking buffer before use. Samples of human plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation as platelets can release PAI-1, which in turn could potentially form a complex with active uPA. Serum and cell culture media at neutral pH may also be used. Functionally active uPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Inactive or complexed uPA will not bind to the PAI-1 and will not be detected by the assay.
Mehr Informationen
Artikelnummer MOIHUPAKT-1
Hersteller Molecular Innovations
Hersteller Artikelnummer HUPAKT-1
Verpackungseinheit 1 kit
Mengeneinheit PAK
Reaktvität Human
Human Gene ID 5328
Wirt Human
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