Swiss ID: P22777
Description: Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of total mouse PAI-1 antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. The concentration level of PAI-1 antigen in mouse plasma was found to be 1.9 ng/ml. The assay measures mouse PAI-1 in the 0.05-50 ng/ml range. Samples giving mouse PAI-1 levels above 50 ng/ml should be diluted in blocking buffer before use. Normal plasma should be applied directly to the plate for best results. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Mouse PAI-1 will bind to the capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will be detected by the assay. Cross-reactivity: 2percent porcine PAI-1, 1.7percent human PAI-1, 0.5percent rabbit PAI-1, 0.3percent rat PAI-1. After appropriate washing steps, anti-mouse PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse PAI-1 in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.