Rat uPA total antigen assay ELISA kit

Rat uPA total antigen assay ELISA kit, 1 kit
Artikelnummer
MOIRUPAKT-TOT-1
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Source: Multiple
Target: Urokinase
Gene: PLAU
Swiss ID: P29598
Description: Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migrations. The sensitive quantitative measurement of total rat uPA antigen in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The assay measures total uPA in the 0.01-10 ng/ml range. Samples giving rat uPA levels above 10 ng/ml should be diluted in blocking buffer before use. Rat uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed uPA will be detected by the assay. After appropriate washing steps, anti-rat uPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of rat uPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified rat uPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
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Artikelnummer MOIRUPAKT-TOT-1
Hersteller Molecular Innovations
Hersteller Artikelnummer RUPAKT-TOT-1
Verpackungseinheit 1 kit
Mengeneinheit PAK
Reaktvität Rat (Rattus)
Human Gene ID 25619
Wirt Rat
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