Source: E. coli
Description: TaqTivate Hot Start Taq MantisGreen Master Mix is an optimized, ready-to-use mix that contains dNTPs, MgCl2, optimized buffers, MantisGreen loading dyes and our TaqTivate recombinant hot start Taq DNA polymerase. Reactions can go directly from PCR tube to gel. The mix is a 2X formulation that requires only the addition of primers, template and water. MantisGreen dual-color loading dyes do not inhibit PCR and make monitoring progress easy during electrophoresis. The dyes run outside the range of typical PCR products and therefore do not obscure visualization. The blue dye runs at 4kb and the yellow dye runs under 25bp. TaqTivate requires a 95C activation time of only two minutes for faster cycling reactions. Chemical modification keeps TaqTivate free of contaminating DNA from biological modifying agents such as antibodies. TaqTivate 2X Master Mix provides robust amplification of templates up to 5kb and reduces non-specific DNA amplification due to primer-dimer formation. Suitable for direct-to-gel Standard Endpoint PCR, Colony PCR, Low Copy PCR, Genotyping, and TA Cloning.