Human PAI-1 (N-terminal cysteine, active fraction)

Human PAI-1 (N-terminal cysteine, active fraction), 0,5 mg
Artikelnummer
MOINTCYSPAI-A-0,5
Verfügbarkeit: wird geladen...
Preis wird geladen...
Molecular Weight: 43000
Storage Buffer: 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; 1mM DTT; pH 6.6
Source: E. coli
Extinction coefficient: 1
Purity: >95% by SDS-PAGE analysis
Gene: SERPINE1
Swiss ID: P05121
Description: Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The active fraction is >95percent pure and >95percent active as determined by titration with HWM tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
Mehr Informationen
Artikelnummer MOINTCYSPAI-A-0,5
Hersteller Molecular Innovations
Hersteller Artikelnummer NTCYSPAI-A-0,5
Verpackungseinheit 0,5 mg
Mengeneinheit FL
Reaktvität Human
Human Gene ID 5054
Datenblatt
×
MSDS Download