Human PAI-1 (N-terminal cysteine LRP non-binding stable mutant)

Human PAI-1 (N-terminal cysteine LRP non-binding stable mutant), 0,5 mg
Artikelnummer
MOINTCYSCPAI-R76E-0,5
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Molecular Weight: 43000
Storage Buffer: 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; 1mM DTT; pH 6.6
Source: E. coli
Extinction coefficient: 1
Purity: >95% by SDS-PAGE analysis
Gene: SERPINE1
Swiss ID: P05121
Description: This stable form of PAI-1 contains an N-terminal cysteine mutation in addition to the four stabilization point mutations. A substitution of Glutamic Acid for Arginine at position 76 greatly decreases the binding of this Human PAI-1 mutant to the low density lipoprotein receptor-related protein (LRP). The protein is supplied in buffer with 1mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
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Artikelnummer MOINTCYSCPAI-R76E-0,5
Hersteller Molecular Innovations
Hersteller Artikelnummer NTCYSCPAI-R76E-0,5
Verpackungseinheit 0,5 mg
Mengeneinheit FL
Human Gene ID 5054
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