Human PAI-1 (N-terminal cysteine mutant, latent fraction)

Human PAI-1 (N-terminal cysteine mutant, latent fraction), 0,5 mg
Artikelnummer
MOINTCYSPAI-L-0,5
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Molecular Weight: 43000
Storage Buffer: 0.1M HEPES; 0.1M NaCl; 1mM DTT; pH 7.4
Source: E. coli
Extinction coefficient: 1
Purity: >95% by SDS-PAGE analysis
Gene: SERPINE1
Swiss ID: P05121
Description: Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The latent fraction is <0.4percent active as determined by titration with HMW tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
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Artikelnummer MOINTCYSPAI-L-0,5
Hersteller Molecular Innovations
Hersteller Artikelnummer NTCYSPAI-L-0,5
Verpackungseinheit 0,5 mg
Mengeneinheit FL
Reaktvität Human
Human Gene ID 5054
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