Human PAI-1 (N-terminal cysteine substrate form stable mutant)

Human PAI-1 (N-terminal cysteine substrate form stable mutant), 0,5 mg
Artikelnummer
MOINTCYSCPAI-T333R-0,5
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Molecular Weight: 43000
Storage Buffer: 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; 1mM DTT; pH 6.6
Source: E. coli
Extinction coefficient: 1
Purity: >95% by SDS-PAGE analysis
Gene: SERPINE1
Swiss ID: P05121
Description: This stable form of PAI-1 contains an N-terminal cysteine mutation in addition to the four stabilization point mutations. A single substitution at position P14 in the reactive center loop produces a PAI-1 that becomes a substrate for proteinases rather than an inhibitor. The protein is supplied in buffer with 1mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
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Artikelnummer MOINTCYSCPAI-T333R-0,5
Hersteller Molecular Innovations
Hersteller Artikelnummer NTCYSCPAI-T333R-0,5
Verpackungseinheit 0,5 mg
Mengeneinheit FL
Human Gene ID 5054
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