Molecular Weight: 70000
Storage Buffer: 0.4M HEPES; 0.1M NaCl; pH 7.4
Source: Insect cell culture
Extinction coefficient: 1,72
Purity: >95% by SDS-PAGE analysis
Swiss ID: P11214
Description: The addition of an arginine to glutamic acid mutation generates mouse tPA that is 100percent single chain and resistant to cleavage by plasmin. The mutation site is R279E on the mature protein and R308E on the complete mRNA sequence (UniProtKB: locus TPA_MOUSE, accession P11214). Recombinantly produced in insect cells.