Molecular Weight: 70000
Storage Buffer: 0.4M HEPES; 0.1M NaCl; pH 7.4
Source: Insect cell culture
Extinction coefficient: 1,9
Purity: >95% by SDS-PAGE analysis
Swiss ID: P00750
Description: This human tPA has an active site serine to alanine mutation which renders it catalytically inactive. The mutation site is S478A on the mature protein and S513A on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). It is >90percent single chain and retains exosite binding as well as other properties of wild type tPA. Recombinantly produced in insect cells.
Identification of a conformationally distinct form of plasminogen activator inhibitor-1, acting as a noninhibitory substrate for tissue- type plasminogen activator
PJ Declerck, M De Mol, DE Vaughan, and D Collen
J. Biol. Chem., Jun 1992; 267: 11693 - 11696.