Molecular Weight: 70000
Storage Buffer: 0.4M HEPES; 0.1M NaCl; pH 7.4
Source: Insect cell culture
Extinction coefficient: 1,72
Purity: >95% by SDS-PAGE analysis
Swiss ID: P11214
Description: This single chain mouse tPA has the active site serine mutated to alanine rendering the enzyme catalytically inactive. The mutation site is S481A on the mature protein and S510A on the complete mRNA sequence (UniProtKB: locus TPA_MOUSE, accession P11214). The tPA still retains exosite binding as well as other biological properties of tPA including but not limited to surface binding to fibrin. This protein is not sterile and should be passed through a 0.22 micron filter before use in cell culture.