Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. MW: 84.4 kDa. Function: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Plays an important role in mitochondrial fission during mitosis Involvement in disease: Encephalopathy due to defective mitochondrial and peroxisomal fission 1 (EMPF1) . Subcellular Location: Cytoplasm, cytosol, Golgi apparatus, Endomembrane system, Peripheral membrane protein, Mitochondrion outer membrane, Peripheral membrane protein, Peroxisome, Membrane, clathrin-coated pit, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.Protein Families: TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family.Tissue Specificity: Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.