Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L(L),partial

Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L(L),partial
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Tag Info: N-terminal 6xHis-tagged. MW: 25.4 kDa. Function: RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene. Subcellular Location: Virion, Host cytoplasm. Protein Families: Rhabdoviridae protein L family.
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Artikelnummer CSB-EP806333VBE-1
Hersteller Cusabio
Hersteller Artikelnummer CSB-EP806333VBE-1
Verpackungseinheit 1 mg
Mengeneinheit FL
Reaktvität Indiana Vesiculovirus
Wirt Escherichia Coli