Best Fluorescence Results with Vectashield Vibrance

VECTASHIELD® Vibrance™ Antifade Mounting Media - New Formulation!

Introducing VECTASHIELD Vibrance Antifade Mounting Media

VECTASHIELD Vibrance Antifade Mounting Media are completely new formulations of curing antifade mounting media for immunofluorescence applications. These newest additions to our VECTASHIELD portfolio were developed with customer feedback to improve on parameters such as ease of use and retention of specific staining intensity over time. VECTASHIELD Vibrance Antifade Mounting Media are new tools to help investigators see more and do more with each experiment.

Human uterine section (FFPE): Stained for desmin (green) and cytokeratin (red) using VectaFluor™ Duet Double Labeling Kit (DK-8828), and vasculature using DyLight® 649 UEA I lectin (DL-1068, purple). Mounted in VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (H-1800, blue).
Human colon section (FFPE) stained for cytokeratin (green) using fluorescein anti-mouse IgG secondary antibody (FI-2000). Mounted in VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (H-1800, blue).

Why VECTASHIELD Vibrance Mounting Media?

  • Superior antifade/anti-photobleaching properties across the spectrum
  • Compatibility with commonly used fluorophores
  • View sections one hour after mounting
  • No tone or autofluorescent background, even after curing
  • Room temperature storage of slides with extended archiving time
  • Minimal bubble formation, even after several weeks storage
  • Curing formulations with choice of counterstain (DAPI) or no counterstain
  • 12 month product expiration date from date of shipping

Plus the same features you have come to expect of the VECTASHIELD brand:

  • Ideal refractive index
  • Easy to use
  • Ready to use, requires no warming
  • No sealing of coverslips required (curing formulations)

VECTASHIELD Vibrance - A Brilliant Solution for Fluorescence Signal Retention!

VECTASHIELD® Vibrance™ Antifade Mounting Media

Easy Application and Use of VECTASHIELD Vibrance

Excellent Antifade Properties Across the Spectrum, with Commonly Used Fluorophores

VECTASHIELD Vibrance Antifade Mounting Media provide excellent protection against fading across the visible spectrum, even under far-red wavelengths. They are compatible with most commercially available fluorophores.

Retention of signal strength after mounting +++ Excellent ++++ Superior

Viewable in as Little as One Hour After Mounting—No Need to Wait 24 hours

Competitor immunofluorescence workflows recommend slides be viewed 24 hours after mounting. VECTASHIELD Vibrance Antifade Mounting Media, however, allow same-day viewing to accelerate discovery without sacrificing signal intensity or retention.

Improved retention of fluorescence with VECTASHIELD Vibrance Antifade Mounting Medium. Serial sections of human colon tissue
(FFPE) stained for cytokeratin (mouse primary antibody AE1/AE3) and detected with fluorescein-conjugated horse anti-mouse IgG
secondary antibody (FI-2000). One hour after mounting with either VECTASHIELD Vibrance (top row) or a competitor mounting
medium (bottom row) the sections were imaged at the intervals indicated. Note the improved retention of fluorescent signal for
sections mounted with VECTASHIELD Vibrance Antifade Mounting Medium.

Extended Archiving of Mounted Sections at Room Temperature...

Archiving fluorescently stained sections at room temperature often leads to signal reduction and dessication of the specimen. Slides mounted with VECTASHIELD Vibrance Antifade Mounting Medium, however, can be stored for weeks at ambient temperature in standard slide boxes with no loss of specific signal intensity or specimen integrity. The stabilizing effect of VECTASHIELD Vibrance Antifade Mounting Medium provides greater flexibility for imaging and storing fluorescently stained samples.

Serial sections of human colon tissue (FFPE) mounted with either VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (H-1800, left image) or competitor mounting medium with DAPI (right image), each stored at room temperature for two weeks and then imaged.

Note absence of bubbles and background in blue spectrum in left image using VECTASHIELD Vibrance Antifade Mounting Medium with DAPI compared with right image, after two weeks at room temperature.

… with No Specimen Obscuring Retraction or Bubble Formation

Another problem commonly observed in immunoflourescence staining is the formation over time of bubbles under the coverslip or shrinking (retraction) of the medium. This phenomenon detracts from the overall quality of the sections and can obscure specific staining. The unique formulation of VECTASHIELD Vibrance Antifade Mounting Medium provides a uniform sheet of medium under the coverslip with essentially no bubble formation or retraction—even after many weeks of storage at room temperature.

Serial sections of human colon tissue (FFPE) stained for cytokeratin (AE1/AE3, mouse primary) followed
by Alexa Fluor™ 488 anti-mouse IgG secondary antibody. Sections were mounted with either VECTASHIELD Vibrance Antifade Mounting Medium (H-1700, left image) or competitor mounting medium (right image) stored at room temperature for two weeks and then imaged.

Note absence of bubbles and background in green spectrum in left image using VECTASHIELD Vibrance Antifade Mounting Medium compared with right image, after two weeks at room temperature. 

Customer Testimonials

Summary of VECTASHIELD® Vibrance™ Advantages Over Competitor Mounting Media

Ordering Info

For further information please contact one of our responsible contact persons (see above right). We are happy to help!

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Ihre Ansprechpartner

  • Petra Vermaeren, PhD

    Produktmanagerin Forschungsprodukte
    +43 1 4893961 30
  • Eliette Weiss

    Kundenservice Forschungsprodukte
    +43 1 4893961 29