VECTASHIELD Vibrance Antifade Mounting Media are completely new formulations of curing antifade mounting media for immunofluorescence applications. These newest additions to our VECTASHIELD portfolio were developed with customer feedback to improve on parameters such as ease of use and retention of specific staining intensity over time. VECTASHIELD Vibrance Antifade Mounting Media are new tools to help investigators see more and do more with each experiment.
Plus the same features you have come to expect of the VECTASHIELD brand:
VECTASHIELD Vibrance - A Brilliant Solution for Fluorescence Signal Retention!
Easy Application and Use of VECTASHIELD Vibrance
VECTASHIELD Vibrance Antifade Mounting Media provide excellent protection against fading across the visible spectrum, even under far-red wavelengths. They are compatible with most commercially available fluorophores.
Competitor immunofluorescence workflows recommend slides be viewed 24 hours after mounting. VECTASHIELD Vibrance Antifade Mounting Media, however, allow same-day viewing to accelerate discovery without sacrificing signal intensity or retention.
Archiving fluorescently stained sections at room temperature often leads to signal reduction and dessication of the specimen. Slides mounted with VECTASHIELD Vibrance Antifade Mounting Medium, however, can be stored for weeks at ambient temperature in standard slide boxes with no loss of specific signal intensity or specimen integrity. The stabilizing effect of VECTASHIELD Vibrance Antifade Mounting Medium provides greater flexibility for imaging and storing fluorescently stained samples.
Note absence of bubbles and background in blue spectrum in left image using VECTASHIELD Vibrance Antifade Mounting Medium with DAPI compared with right image, after two weeks at room temperature.
Another problem commonly observed in immunoflourescence staining is the formation over time of bubbles under the coverslip or shrinking (retraction) of the medium. This phenomenon detracts from the overall quality of the sections and can obscure specific staining. The unique formulation of VECTASHIELD Vibrance Antifade Mounting Medium provides a uniform sheet of medium under the coverslip with essentially no bubble formation or retraction—even after many weeks of storage at room temperature.
Note absence of bubbles and background in green spectrum in left image using VECTASHIELD Vibrance Antifade Mounting Medium compared with right image, after two weeks at room temperature.
For further information please contact one of our responsible contact persons (see above right). We are happy to help!