SZABO-SCANDIC presents: Immunostep - The right choice for Flow Cytometry

Neu bei SZABO-SCANDIC

Das spanische Unternehmen ImmunoStep wurde 2001 gegründet. Diese junge und dynamische Firma entwickelt Reagenzien, Kits und Geräte für Durchflusszytometrie. Diese Produkte werden sowohl in der Forschung als auch in der Diagnostik mit entsprechender CE-IVD Markierung eingesetzt. Um beste Qualität und Produktionssicherheit gewährleisten zu können arbeitet Immunostep nach den ISO Normen 9001 und 13485 und verfügt weiters über zehn internationale Patente im Bereich Diagnostik.

 

Szabo-Scandic - Ihr exklusiver Distributor für den österreichischen Markt!

 

Nachfolgend möchten wir Ihnen die verschiedenen Produktbereiche von Immunostep näher vorstellen.


Primary Antibodies

Kappa & Lambda

 

Human and Mouse Immunology

 

Multi-Color Reagents

 

Monoclonal & polyclonal Antibodies for Western Blot

 


Apoptosis Tools

FITC Annexin V Apoptosis Detection Kit, CE-IVD (Product Nr. IMSANXVF)

 

MitoStepMitochondrial membrane potencial assay (Product Nr. IMSMITO)

 


Kits

(Click links for further information)

ExoStep

Kit for detection and characterization of exosomes from plasma, urine or cell culture (Product Nr. IMSExoS)

 

BasoStep

Quantitative determination of degranulation of basophilic granulocytes in heparinized human whole blood (Product Nr. IMSBSTP)

 

PNH

Screening kit for paroxysmal nocturnal hemoglobinuria (PNH) (Product Nr. IMSPNH)

 

FMHStep 

Kit for quantification of fetomaternal hemorrhage (Product Nr. IMSRHD)

 

Mesenchymal cell kit 

Kit for identification of human multipotent mesenchymal stromal cells (Product Nr. IMSMCK)

 

Stem cell kit 

Kit designed for quantitative determination of hematopoietic stem cells (HSCs) CD34 positive, CE-IVD (Product Nr. IMSSCK)

 

XMStep

Kit for determination of transplantational cross-match (Product Nr. IMSFCXM)

 

ThromboStep

Kit designed for detection and quantification of platelet-associated immunoglobulin, CE-IVD (Product Nr. IMSFCXM)

 


Custom services

Peptide production service – design, synthesis, modification

 

Protein service  expression, purification, lyophilisation

 

Monoclonal and polyclonal antibody development service

 

Discovery biology development of customized assays

 

Specialized antibody services conjugation, hybridoma isotyping, antibody purification, antibody fragmentation, bi-specific monoclonal antibodies 

 


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ExoStep (# IMSExoS)

Description: Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment - the multivesicular body, with the plasma membrane. They are thought to provide a means of intercellular communication and transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.

The kit is a simple immunobead assay for isolation/detection of exosomes, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.

 

ExoStep Cell Culture - IMSExoS-25-C

ExoStep Plasma - IMSExoS-25-P

ExoStep Urine - IMSExoS-25-U

 

 

 

  • Tested Applications: FCM
  • Species Reactivity: Human
  • Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • Regulatory Status: RUO
  • Storage Instruction: Store in the dark at 2-8 ºC

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BasoStep (# IMSBSTP)

Description: BasoStep is intended for flow cytometry test of IgE-mediated allergic reactions by the analysis of CD63 antigen surface on basophils upon allergen stimulation.

 

Kit includes:


  • Stimulation Buffer: containing calcium, heparin and IL-3 (3 vials lyophilized)
  • Stimulation Control: containing fMLP and anti-IgE. (1 vial lyophilized)
  • Staining Reagent: antibody monoclonal combination

IMSBSTP100 - CD63-FITC/CD203c-PE/HLA-DR-PerCP/CD123-APC (1 vial 100 test, liquid)

 

IMSBSTP2-100 - CD63-FITC/CD123-PE/HLA-DR-PerCP (1 vial 100 test, liquid)

 

IMSBSTP3-100 - CD63-FITC/CD123-PE/HLA-DR-APC (1 vial 100 test, liquid)

  • Tested Applications: FCM
  • Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • Regulatory Status: RUO
  • Recommended Usage: Kit for the quantitative determination of the degranulation of basophilic granulocytes in heparinized human whole blood.
  • Storage Instruction: Store in the dark at 2-8 °C!

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PNH Kit (# IMSPNH)

Description: Kit for Paroxysomal Nocturnal Hemoglobinuria (PNH) with three-color direct immunofluorescence reagent for use in flow cytometry to evaluate the possible loss of expression of the glycosylphosphatidylinositol (GPI) anchor (GPI-anchored proteins) molecule on neutrophils and on monocytes. Kit includes monoclonal antibodies combination: CD157/CD45/CD64.

  • Tested Applications: FCM
  • Species Reactivity: Human
  • Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • Regulatory Status: RUO
  • Recommended Usage: Kit is a screening Kit for Paroxysomal Nocturnal Hemoglobinuria (PNH)
  • Storage Instruction: Store in the dark at 2-8 °C

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FMHStep (# IMSRHD)

Description: The anti-RhD monoclonal antibody binds to the surface of red blood cells (RBC) of RhD positive individuals to identify these cells, allowing the quantitation of Fetomaternal hemorrhage (FMH), which may occur during pregnancy or at delivery and lead to immunisation to the D antigen if the mother is D negative and the baby D positive.

  • Tested Applications: FCM
  • Species Reactivity: Human
  • Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • Regulatory Status: RUO
  • Recommended Usage: Anti-RhD, Monoclonal Antibody for quantitation of Fetomaterna Hemorrhage
  • Storage Instruction: Store in the dark at 2-8 °C

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Mesenchymal Cell Kit (# IMSMCK)

Description: The MSC kit is based on the phenotypic signature described by The Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy (ISCT).

Kit includes vials, A, B and C:

  • Vial A: CD90 FITC, CD105 PE, CD45 PerCP, CD19 PerCP, HLA-DR PerCP, CD14 PerCP, CD34 PerCP, CD29 APC
  • Vial B: CD44 FITC, CD166 PE, CD45 PerCP, CD19 PerCP, HLA-DR PerCP, CD14 PerCP, CD34 PerCP, CD73 APC
  • Vial C: Isotype control IgG1 FITC, Isotype control IgG1 PE, Isotype control IgG1 PerCP, Isotype control IgG1

In addition the kit includes four aliquots of antibody to perform the compensation: CD90 FITC, CD166 PE, CD44 PerCP and CD29 APC.

  • Tested Applications: FCM
  • Species Reactivity: Human
  • Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • Regulatory Status: RUO
  • Recommended Usage: Kit for identification of human multipotent mesenchymal stromal cells.
  • Storage Instruction: Store in the dark at 2-8 °C

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Stem Cell Kit (# IMSSCK)

Description: Kit allows the identification of CD34+ cells and link together their number with the identified known number of spheres discriminating between viable and non-viable cells and discarding false positives produced by non-specific staining of the isotype. Kit includes a vial of CD45 / CD34, a CD45/ IgG1 isotype control vial, counting tubes, a vial of 7-AAD and a bottle of lysis solution 10X.

  • Tested Applications: FCM
  • Species Reactivity: Human
  • Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • Regulatory Status: IVD-CE
  • Recommended Usage: kit designed for quantitative determination of hematopoietic stem cells (HSCs) CD34 positive
  • Storage Instruction: Store in the dark at 2-8 °C

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XMStep (# IMSFCXM)

Description: XMStep kit is intended for determination of presence of anti-HLA antibodies preformed in a patient's serum against lymphocytes of a possible donor. The kit includes a mouse anti-human IgG monoclonal antibody conjugated with a flurochrome (FITC).

  • Tested Applications: FCM
  • Species Reactivity: Human
  • Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • Regulatory Status: RUO
  • Storage Instruction: Store in the dark at 2-8 °C

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ThromboStep (# IMSTBS)

Description: Flow cytometry detection of autoantibodies reacting with specific platelet receptors. The kit contains an R-phycoerythrin (R-PE) conjugated monoclonal antibody, which recognizes a platelet specific antigen. The kit also contains polyclonal antibodies conjugated whit fluorescein isothiocyanate (FITC) against total human immunoglobulins, human IgA, IgM and IgG. The kit contains a polyclonal antibody conjugated with FITC as a negative control.

  • Tested Applications: FCM
  • Species Reactivity: Human
  • Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • Regulatory Status: IVD-CE
  • Recommended Usage: Kit designed for detection and quantitation of platelet associated immunoglobulin using flow cytometry
  • Storage Instruction: Store in the dark at 2-8 °C

Für weitere Informationen kontaktieren Sie bitte einen unserer dafür zuständigen Ansprechpartner (siehe rechts oben).
Wir beraten Sie gerne!

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Ihre Ansprechpartner

  • Petra Koudelkova, PhD

    Produktmanagerin Forschungsprodukte
    +43 1 4893961 30
  • Eliette Weiss

    Kundenservice Forschungsprodukte
    +43 1 4893961 29