AKT1 antibody (AKT1/2552)

Clone Name: AKT1/2552

Light Chain: Kappa

Application Note: WB: 1-2μg/ml. IHC-P: 1-2μg/ml for 30 min at RT. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 56

Form: Liquid

Buffer (with preservative): 10mM PBS, 0.05% BSA, 0.05% Sodium azide.

Concentration: 0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot ID: P31749

Antigen Species: Human

Immunogen: Recombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary)

Purification: Protein A/G purified

Conjugation: Unconjugated

Full Name: AKT serine/threonine kinase 1