Anti-Mouse TNF alpha In Vivo Antibody - Ultra Low Endotoxin (TN3-19.12 )

Target: TNF alpha.

Clone: TN3-19.12.

Specificity: Anti-TNF alpha In Vivo Antibody - Ultra Low Endotoxin (TN3-19.12 ) recognizes Mouse TNFa.

Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells.

Background: The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity. It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites. TNF-alpha is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-alpha secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-alpha has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-alpha.

Immunogen: Recombinant mouse TNFalpha.

Concentration: ≥ 2.0 mg/ml.

Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Purity: >98% by SDS-PAGE and HPLC.

Endotoxin: ≤ 0.75 EU/mg as determined by the LAL method.

Aggregation: Aggregation level ≤ 1%.

Use: Products are for research use only. Not for use in diagnostic or therapeutic procedures.