GAPDHS antibody (Hs-8) (FITC)

Clone Name: Hs-8

Application Note: FACS: 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 44

Form: Liquid

Buffer (with preservative): TBS, 15mM Sodium azide.

Concentration: Batch dependent (Please refer to the vial label for the specific concentration.)

Background: This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq, Jul 2008]

Uniprot ID: O14556

Antigen Species: Human

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Purification: Purified by size-exclusion chromatography

Conjugation: FITC

Full Name: glyceraldehyde-3-phosphate dehydrogenase, spermatogenic