Heparan Sulfate antibody (10E4)

Clone Name: 100000

Light Chain: Kappa

Application Note: FACS: 1:100-1:200 (0.5-1μg labels ~10⁵ cells). ELISA: 1:100-1:500. IHC: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Specificity/Sensitivity: Recognizes an epitope present in many types of human heparan sulfate. The epitope includes N-sulfated glucosamine residues that are critical for the reactivity of the antibody. Does not react with hyaluronan, chondroitin sulfate, dermatan sulfate, keratan sulfate or DNA. Reactivity with most heparan sulfates is nearly completely abolished after treatment of the glycosaminoglycan with bacterial heparitinase (Flavobacterium heparinum, EC 4.2.2.8).

Form: Liquid

Buffer (with preservative): PBS pH7.4, 0.02% Sodium azide.

Concentration: Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species: Human

Immunogen: Liposome-incorporated membrane heparan sulfate proteoglycan from human fetal lung fibroblasts.

Purification: Purified by tangential ultrafiltration

Conjugation: Unconjugated