Laminin alpha 3B antibody (F7)

Clone Name: F7

Product Description: Laminins, which consist of three subunits called alpha, beta and gamma chains, are major cell-adhesive components of extracellular matrix, especially basement membranes (BMs). The laminin family is constituted of over 15 isoforms, and each member is expressed in a tissue-specific manner and plays a differential role in each tissue. In the case of laminin alpha 3 chain, there are two splicing variants, the truncated form alpha 3A and the full-length alpha 3B. Laminin-3B32 (Lm3B32) (formerly, laminin-5B) is composed of alpha 3B, beta 3 and gamma 2 chains and less widely expressed than laminin-3A32. Although laminin-3B32 shows higher cell adhesion activity than laminin-3A32 in vitro, differences of biological functions between two laminins remain to be clarified. Recent studies identified laminin-3B11 (Lm3B11) as a new alpha 3B-type laminin. Laminin-3B11 is localized in vascular basement membranes in normal tissues, but this expression is down-regulated in cancer tissues.Laminin-3B11 stimulates microvascular endothelial cells to extend lamelliopodial protrusions. This antibody (clone F7) detects laiminin-3B32 in the basement membranes of normal epithelial tissues and of relatively benign or differentiated carcinomas and laminin-3B11 in normal vascular basement membranes. This antibody is a powerful tool to detect laminin alpha 3B isoform and investigate its fundamental functions in epithelial and vascular basement membranes.

Application Note: WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Specificity/Sensitivity: This antibody is specific for alpha 3B chain

Form: Liquid

Buffer (with preservative): Ascites, no preservative.

Concentration:

Notes: For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Antigen Species: Human

Immunogen: Recombinant human laminin-alpha 3B N-term ~190 kDa fragment

Purification: Unpurified

Conjugation: Unconjugated