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HSP70/HSC70 Antibody: Dylight 633, Monoclonal

HSP70/HSC70 Antibody: Dylight 633, Monoclonal
405,15 €
Artikelnummer: STRSMC-106B-DY633

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

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HSP70/HSC70 Antibody: Dylight 633, Monoclonal, Clone: BB70, Host: Mouse, Isotype: IgG2a

Details

Mouse Anti-Chicken HSP70/HSC70 Monoclonal IgG2a
Target: HSP70/HSC70
Purification: Protein G Purified
Specificity: Detects ~72 (HSP) and ~73kDa (HSC).
Conjugate: Dylight 633
Immunogen Species: Chicken
Cellular Location: Cytoplasm
Immunogen: Chicken HSP70/HSP90 complex
Scientific Background: HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
Reference: 1. Zho J. (1998) Cell 94 : 471-480.2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17.3. Rothman J. (1989) Cell 59: 591 -601.4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.5. Bork P., Sander C. & Valencia A. (1992) Proc. Nat Acad. Sci. USA 89: 7290-7294.6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.7. Smith D.F., et al, (1993) Mol. Cell. Biol. 13(2): 869-876.8. Prapapanich V., et al. (1996) Mol. Cell. Biol. 16(11):6200-6207.9. Fernandez-Funez et al., (2000) Nature 408(6808): 101-106.

Zusatzinformation

Artikelnummer STRSMC-106B-DY633
Hersteller Stressmarq Biosciences
Herstellernummer SMC-106B-DY633
Verpackungseinheit 200 µg
Mengeneinheit FL
Reaktivität Beluga, Chicken, Cow (Bovine), Dog (Canine), Fish, Frog (Xenopus Laevis), Fruit Fly (Drosophila Melanogaster), Guinea Pig, Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Yeast
Klonalität Monoclonal
Wirt / Host Mouse
Methode Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Isotype IgG2
Gene ID NCBI (externer Link)
Datenblatt Download
MSDS Download