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DNMT1 Antibody: Dylight 405, Monoclonal

DNMT1 Antibody: Dylight 405, Monoclonal
364,85 €
Artikelnummer: STRSMC-201D-DY405

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

Kostenlose Lieferung innerhalb Österreichs!
DNMT1 Antibody: Dylight 405, Monoclonal, Clone: 4G11-C7, Host: Mouse, Isotype: IgG1

Details

Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa
Target: DNMT1
Purification: Protein G Purified
Specificity: Detects ~180kDa. It will cross-react with mouse DNMT1.
Conjugate: Dylight 405
Immunogen Species: Human
Cellular Location: Nucleus
Immunogen: Raised against a partial protein corresponding to amino acids 620-950 of human DNMT1
Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
Reference: 1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983.2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291.3. Xie S., et al. (1999) Gene 236: 87-95.4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257.5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382.6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.

Zusatzinformation

Artikelnummer STRSMC-201D-DY405
Hersteller Stressmarq Biosciences
Herstellernummer SMC-201D-DY405
Verpackungseinheit 100 µg
Mengeneinheit FL
Reaktivität Fish, Human, Mouse (Murine), Zebrafish
Klonalität Monoclonal
Wirt / Host Mouse
Methode Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunoprecipitation, Western Blotting
Isotype IgG1
Gene ID NCBI (externer Link)
Datenblatt Download
MSDS Download