• Sie sehen derzeit Listenpreise. Um Ihren Vorteilspreis zu sehen, melden Sie sich mit Ihrem Kundenkonto an.

Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa Dylight 633

Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa Dylight 633
335,80 €
Artikelnummer: STRSMC-200D-DY633

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

Kostenlose Lieferung innerhalb Österreichs!
Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa Dylight 633, Clone: 60B1220.1

Details

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

2 µg/ml of SMC-200 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Zusatzinformation

Artikelnummer STRSMC-200D-DY633
Hersteller Stressmarq Biosciences
Herstellernummer SMC-200D-DY633
Verpackungseinheit 100 µg
Mengeneinheit FL
Reaktivität Fish, Human, Mouse (Murine), Zebrafish
Klonalität Monoclonal
Wirt / Host Mouse
Methode Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunoprecipitation, Western Blotting
Isotype IgG
Gene ID NCBI (externer Link)
Datenblatt Download
MSDS Download