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HSP60 Antibody: Dylight 633, Monoclonal

HSP60 Antibody: Dylight 633, Monoclonal
323,48 €
Artikelnummer: STRSMC-110B-DY633

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

Kostenlose Lieferung innerhalb Österreichs!
HSP60 Antibody: Dylight 633, Monoclonal, Clone: LK1, Host: Mouse, Isotype: IgG1

Details

Mouse Anti-Human HSP60 Monoclonal IgG1
Target: HSP60
Purification: Protein G Purified
Specificity: Detects ~60kDa.
Conjugate: Dylight 633
Immunogen Species: Human
Cellular Location: Mitochondrion, Mitochondrion Matrix
Immunogen: Recombinant human HSP60
Scientific Background: In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
Reference: 1. Hartl, F.U. (1996) Nature 381: 571-579.2. Bukau, B. and Horwich, A.L. (1998) Cell 92: 351-366.3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295: 1852- 1858.4. Jindal, S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283.5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71.6. Itoh, H. et al. (2002) Eur. J. Biochem. 269: 5931-5938.7. Gupta, S. and Knowlton, A.A. J. Cell Mol Med. 9: 51-58.8. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11: 116-128.9. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297.10. Gao, Y.L., et al (1995) J. of Immunology 154: 3548-3556.11. Neuer, A., et al (1997) European Society for Human Reproduction and Embryology 12(5):925-929. 12. Bason, C., et al (2003) Lancet 362(9400): 1971-1977.

Zusatzinformation

Artikelnummer STRSMC-110B-DY633
Hersteller Stressmarq Biosciences
Herstellernummer SMC-110B-DY633
Verpackungseinheit 200 µg
Mengeneinheit FL
Reaktivität Chicken, Cow (Bovine), Dog (Canine), Frog (Xenopus Laevis), Fruit Fly (Drosophila Melanogaster), Guinea Pig, Hamster, Human, Monkey (Primate), Mouse (Murine), Pig (Porcine), Plant, Rabbit, Rat (Rattus), Sheep (Ovine), Silkworm
Klonalität Monoclonal
Wirt / Host Mouse
Methode ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blotting
Isotype IgG1
Gene ID NCBI (externer Link)
Datenblatt Download
MSDS Download