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Mouse Anti-Human HSP70/HSC70 Monoclonal IgG1 Dylight 488

Mouse Anti-Human HSP70/HSC70 Monoclonal IgG1 Dylight 488
405,80 €
Artikelnummer: STRSMC-104B-DY488

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

Kostenlose Lieferung innerhalb Österreichs!
Mouse Anti-Human HSP70/HSC70 Monoclonal IgG1 Dylight 488, Clone: N27F3-4

Details

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

1 µg/ml of SMC-104 was sufficient for detection of HSP70/HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Zusatzinformation

Artikelnummer STRSMC-104B-DY488
Hersteller Stressmarq Biosciences
Herstellernummer SMC-104B-DY488
Verpackungseinheit 200 µg
Mengeneinheit FL
Reaktivität Beluga, Caenorhabditis Elegans, Chicken, Cow (Bovine), Dog (Canine), Fish, Frog (Xenopus Laevis), Fruit Fly (Drosophila Melanogaster), Guinea Pig, Hamster, Human, Monkey (Primate), Mouse (Murine), Pig (Porcine), Plant, Rabbit, Rat (Rattus), Sheep (Ovine)
Klonalität Monoclonal
Wirt / Host Mouse
Methode Flow Cytometry, Immuno Electron Microscopy (IEM), Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Isotype IgG
Gene ID NCBI (externer Link)
Datenblatt Download
MSDS Download