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Mouse Anti-Human HSP90 complex Monoclonal IgM Dylight 488

Mouse Anti-Human HSP90 complex Monoclonal IgM Dylight 488
483,91 €
Artikelnummer: STRSMC-109B-DY488

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

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Mouse Anti-Human HSP90 complex Monoclonal IgM Dylight 488, Clone: 8D3

Details

HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7).

Goat anti-mouse IgM was used to bind 25 µl of protein G-Sepharose. SMC-109 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 µl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including HSP90, HSP70 and Hop.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Zusatzinformation

Artikelnummer STRSMC-109B-DY488
Hersteller Stressmarq Biosciences
Herstellernummer SMC-109B-DY488
Verpackungseinheit 200 µg
Mengeneinheit FL
Reaktivität Human, Mouse (Murine), Rabbit, Rat (Rattus)
Klonalität Monoclonal
Wirt / Host Mouse
Methode Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Isotype IgM
Gene ID NCBI (externer Link)
Datenblatt Download
MSDS Download