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Mouse Anti-Mouse HIF1 alpha Monoclonal IgG1 Dylight 405

Mouse Anti-Mouse HIF1 alpha Monoclonal IgG1 Dylight 405
317,54 €
Artikelnummer: STRSMC-184D-DY405

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

Kostenlose Lieferung innerhalb Österreichs!
Mouse Anti-Mouse HIF1 alpha Monoclonal IgG1 Dylight 405, Clone: ESEE122

Details

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).

1 µg/ml of SMC-184 was sufficient for detection of HIF1α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Zusatzinformation

Artikelnummer STRSMC-184D-DY405
Hersteller Stressmarq Biosciences
Herstellernummer SMC-184D-DY405
Verpackungseinheit 100 µg
Mengeneinheit FL
Reaktivität Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
Klonalität Monoclonal
Wirt / Host Mouse
Methode ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blotting
Isotype IgG
Gene ID NCBI (externer Link)
Datenblatt Download
MSDS Download