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CaMKII Antibody: Dylight 350, Monoclonal

CaMKII Antibody: Dylight 350, Monoclonal
355,30 €
Artikelnummer: STRSMC-125D-DY350

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

Kostenlose Lieferung innerhalb Österreichs!
CaMKII Antibody: Dylight 350, Monoclonal, Clone: 22B1, Host: Mouse, Isotype: IgG1

Details

Mouse Anti-Rat CaMKII Monoclonal IgG1
Target: CaMKII
Purification: Protein G Purified
Specificity: Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
Conjugate: Dylight 350
Immunogen Species: Rat
Cellular Location: Cytoplasm, Mitochondrion, Nucleus, Cell Junction, Synapse, Presynaptic Cell Membrane
Immunogen: Synthetic peptide
Scientific Background: CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Reference: 1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013.2. Barria A. et al. (1997) Science 276: 2042–2045.3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798.4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808.5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479.6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102.7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292.8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12.9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510.10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692.11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244.12. Shen K. and Meyer Y. (1999) Science 284: 162-167.

Zusatzinformation

Artikelnummer STRSMC-125D-DY350
Hersteller Stressmarq Biosciences
Herstellernummer SMC-125D-DY350
Verpackungseinheit 100 µg
Mengeneinheit FL
Reaktivität Human, Mouse (Murine), Rat (Rattus)
Klonalität Monoclonal
Wirt / Host Mouse
Methode ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Isotype IgG1
Gene ID NCBI (externer Link)
Datenblatt Download
MSDS Download