PTEN antibody (PTEN/2110)

Clone Name: PTEN/2110

Light Chain: Kappa

Application Note: IHC-P: 1-2μg/ml for 30 minutes at RT. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 47

Form: Liquid

Buffer (with preservative): 10mM PBS, 0.05% BSA, 0.05% Sodium azide.

Concentration: 0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Background: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]

Uniprot ID: P60484

Antigen Species: Human

Immunogen: Recombinant full-length human PTEN protein

Purification: Protein A/G purified

Conjugation: Unconjugated

Full Name: phosphatase and tensin homolog