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HSF1 Antibody: Dylight 594, Monoclonal

HSF1 Antibody: Dylight 594, Monoclonal
284,24 €
Artikelnummer: STRSMC-118D-DY594

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

Kostenlose Lieferung innerhalb Österreichs!
HSF1 Antibody: Dylight 594, Monoclonal, Clone: 10H8, Host: Rat, Isotype: IgG1

Details

Rat Anti-Mouse HSF1 Monoclonal IgG1
Target: HSF1
Purification: Protein G Purified
Specificity: Detects ~85kDa (unstressed cell lysates), and~95kDa (heat shocked cell lysates).
Conjugate: Dylight 594
Immunogen Species: Mouse
Cellular Location: Cytoplasm, Nucleus
Immunogen: Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
Scientific Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
Reference: 1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934.2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82.3.Tanaka KI et al. (2007). JBC Papers Online Manuscript M704081200.4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796.5. McMillan D. R., Xiao X., Shao L., Graves K., and Benjamin I. J. (1998) J Bio Chem 273: 7523-7528.6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774.7. Fiorenza M.T., Farkas T., Dissing M., Kolding D. and Zimarino V. (1995) Nucleic Acids Res. 23 (3):467-474.8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293.9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397.10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334- 1343.11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.

Zusatzinformation

Artikelnummer STRSMC-118D-DY594
Hersteller Stressmarq Biosciences
Herstellernummer SMC-118D-DY594
Verpackungseinheit 100 µg
Mengeneinheit FL
Reaktivität Cow (Bovine), Guinea Pig, Hamster, Human, Monkey (Primate), Mouse (Murine), Rabbit, Rat (Rattus)
Klonalität Monoclonal
Wirt / Host Rat
Methode ELISA, Gel Shift, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Isotype IgG1
Gene ID NCBI (externer Link)
Datenblatt Download
MSDS Download