ZAP70 antibody (ZAP70/2035)

Clone Name: ZAP70/2035

Light Chain: Kappa

Application Note: ICC/IF: 1-2μg/ml. IHC-P: 1-2μg/ml for 30 minutes at RT. FACS: 1-2μg/10⁶ cells. ELISA: 2-4μg/ml (for coating). *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Calculated MW: 70

Form: Liquid

Buffer (with preservative): 10mM PBS, 0.05% BSA, 0.05% Sodium azide.

Concentration: 0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Background: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot ID: P43403

Antigen Species: Human

Immunogen: Recombinant fragment of human ZAP70 protein (around aa 247-382) (exact sequence is proprietary)

Purification: Protein A/G purified

Conjugation: Unconjugated

Full Name: zeta chain of T cell receptor associated protein kinase 70