The IncuCyte® NeuroBurst Orange is a lentiviral based live-cell neuronal labeling reagent driven by a synapsin promoter, resulting in the long term expression of a genetically encoded orange fluorescent calcium indicator (mRuby-based) in neuronal cells. The NeuroBurst Orange Reagent ensures highly efficient, yet non-disruptive labeling of primary or iPSC-derived neurons over days and weeks, and enables the kinetic quantification of neuronal activity and functional connectivity in the presence or absence of astrocytes.