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Active rabbit tPA functional assay ELISA kit

Active rabbit tPA functional assay ELISA kit
1.916,39 €
Artikelnummer: MOIRbTPAKT-5

Verfügbarkeit: Regellieferzeit: Innerhalb 1 - 2 Wochen

Kostenlose Lieferung innerhalb Österreichs!
Active rabbit tPA functional assay ELISA kit, 5 kits; Host: Rabbit

Details

Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases. The sensitive quantitative measurement of functionally active rabbit tPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. In-house testing of pooled normal rabbit plasma in citrate indicates a tPA concentration of 0.5-1 ng/ml. The assay measures active tPA in the 0.05-20 ng/ml range. Samples giving rabbit tPA levels above 20 ng/ml should be diluted in blocking buffer before use. Samples of plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1, which in turn could potentially form a complex with active tPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the 10X TBS provided in the kit. Serum and cell culture media at neutral pH may also be used. Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, anti-rabbit tPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active tPA in the samples. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Zusatzinformation

Artikelnummer MOIRbTPAKT-5
Hersteller Molecular Innovations
Herstellernummer RbTPAKT-5
Verpackungseinheit 5 kits
Mengeneinheit KAR
Reaktivität Rabbit
Klonalität Keine Herstellerangabe
Wirt / Host Rabbit
Methode Keine Herstellerangabe
Isotype Keine Herstellerangabe
Gene ID NCBI (externer Link)
Datenblatt Auf Anfrage
MSDS Download