THUNDER Total AKT pan TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Total AKT pan in cell lysates.

Components: Eu-Total AKT (5 µL), Acc-Total AKT (20 µL), AKT control lysate (100 µL), 5X Lysis Buffer 1 (1 mL), 10X TR-FRET Detection buffer (50 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Total AKT pan.

Assay Target Class: Kinase.

SwissProt: P31749 , P31751 , Q9Y243.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).